What is the catalog number for IL-22, pegylated Recombinant Protein?

The catalog number for IL-22, pegylated Recombinant Protein is 500-026.

Is IL-22, pegylated Recombinant Protein in stock?

IL-22, pegylated Recombinant Protein is currently made to order / not held in immediate stock. Contact us for lead time.

IL-22, pegylated Recombinant Protein

Mono-pegylated (with 20 kDa PEG)recombinant human interleukin 22 is one polypeptide chain containing 147 amino, an additional Ala at N-terminus and one molecule of PEG 20 kDa at its N-terminus, having an expected molecular mass of ~ 36 kDa as determined by MS. However due to enlarged hydrodymanic volume it runs on the SDS-PAGE as 50 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein. It was prepared similarly to that described by L. Niv-Spector et al. Protein Eng Des Sel. (PEDS) 25:397-404 (2012).

Product Specifications

CAS Number

9000-83-3

Synonyms

I-TIF

NCBI Gene ID

50616

UniProt

Q9GZX6

Accession Number

NP_065386.1

Accession Number mRNA

NM_020525.4

Host

E. coli

Origin Species

Human

Species Reactivity

Human

Tag

PEG

Sequence

AAPISSHCRLDKSNFQQPYITNRTFMLAKEASLADNNTDVRLIGEKLFHGVSMSERCYLMKQVLNFTLEEVLFPQSDRFQPYMQEVVPFLARLSNRLSTCHIEGDDLHIQRNVQKLKDTVKKLGESGEIKAIGELDLLFMSLRNACI

Detection Range

Recombinantr human IL-22 is biologically active when compared to standard using STAT3 phosphorylation assay in HepG2 cells. Its activity in vitro is ~ 10% compared to the non-pegylated mIL22.

Endotoxin

< 0.1 ng/µg of protein (<1EU/µg)

Purity

> 98.0% as determined by Gel filtration and SDS-PAGE gel.

Length

147

Form

Lyophilized

Reconstitution

It is recommended to reconstitute the lyophilized pegylated mouse IL22 in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted with other aqueous solutions.