IL-11

Interleukin 11 (IL-11), also known as adipogenesis inhibitory factor (AGIF), has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. IL-11 exerts its biological activities through binding to a specific high-affinity receptor complex consisting of an IL-11 receptor chain and gp130.

Product Specifications

Synonyms

IL11; AGIF; IL-11

NCBI Gene ID

3589

UniProt

P20809

Accession Number

NP_000632.1

Accession Number mRNA

NM_000641

Chromosomal Location

19q13.3-q13.4

Reactivity

Anti-Human

Cross Reactivity

Human

Target Antigen

Recombinant mouse IL-11

Applications

ELISA, N, IHC

Purification Method

Protein G chromatography

Assay Protocol

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Bioactivity

Immunostaining: This antibody stained human brain stroke (including control cortex and stroke core areas) tissue. The primary antibody was incubated at 2.5 µg/ml overnight at 4°C. This was followed by a fluorophore conjugated secondary antibody. Neutralization: To yield one-half of the maximal inhibition [ND50] of the biological activity of human IL-11 (5 ng/ml), a concentration of 2.4-3.6 µg/ml of this antibody is required.

Form

Lyophilized from PBS

Buffer

PBS

Reconstitution

Water

Storage Conditions

The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C.