IL-2
IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response.
Product Specifications
Synonyms
IL2; IL-2; TCGF; lymphokine
NCBI Gene ID
3558
UniProt
P60568
Accession Number
NP_000577
Accession Number mRNA
NM_000586
Chromosomal Location
4q26-q27
Reactivity
Human
Cross Reactivity
Human
Sequence
MAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLHYKDPAANKARKEAELAAATAEQ
Assay Protocol
The lyophilized IL-2 should be reconstituted in 50mM acetic acid to a concentration not less than 100µg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use.
Endotoxin
< 0.1 ng per µg of IL-2
Purity
> 98% by SDS-PAGE
Bioactivity
The ED50 as determined by the dose-dependent stimulation of proliferation of murine D10G4.1 cells is in the range of 0.1-0.5 ng/ml.
Length
156
Form
Lyophilized
Buffer
50mM acetic acid
Reconstitution
50mM acetic acid
Molecular Weight
15.5 kDa
Storage Conditions
The lyophilized IL-2, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted IL-2 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Host or Source
E. coli
N Terminal Sequence
APTSSSTK and MAPTSSST
Curated Selection
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