IL-2

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response.

Product Specifications

Synonyms

IL2; IL-2; TCGF; lymphokine

NCBI Gene ID

3558

UniProt

P60568

Accession Number

NP_000577

Accession Number mRNA

NM_000586

Chromosomal Location

4q26-q27

Reactivity

Human

Cross Reactivity

Human

Sequence

MAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLHYKDPAANKARKEAELAAATAEQ

Assay Protocol

The lyophilized IL-2 should be reconstituted in 50mM acetic acid to a concentration not less than 100µg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use.

Endotoxin

< 0.1 ng per µg of IL-2

Purity

> 98% by SDS-PAGE

Bioactivity

The ED50 as determined by the dose-dependent stimulation of proliferation of murine D10G4.1 cells is in the range of 0.1-0.5 ng/ml.

Length

156

Form

Lyophilized

Buffer

50mM acetic acid

Reconstitution

50mM acetic acid

Molecular Weight

15.5 kDa

Storage Conditions

The lyophilized IL-2, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted IL-2 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.