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IL-1 receptor antagonist

IL-1 receptor antagonist (IL-1ra) was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells. In mouse, IL-1RII does not bind IL-1ra.

Product Specifications

Synonyms

IL1RN; DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA

NCBI Gene ID

3557

UniProt

P18510

Accession Number

NP_776214.1

Accession Number mRNA

NM_173842.2

Chromosomal Location

2q14.2

Reactivity

Anti-Human

Cross Reactivity

Human

Target Antigen

Human recombinant protein of IL-1ra

Clone

(#2V23)

Applications

WB

Purification Method

Protein G chromatography

Assay Protocol

Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

Bioactivity

WB: 1:500-2000

Form

Lyophilized

Buffer

PBS

Reconstitution

PBS

Storage Conditions

Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

Host or Source

Rat

Isotype

IgG2

Frequently Asked Questions

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