IGF-1
IGF-1 is a small protein secreted mainly but not solely by the liver and circulating in blood mostly as a complex with various IGF binding proteins in the blood. It has growth-regulating, insulin-like, and mitogenic activities and it is secreted in response to growth hormone stimulation. Recombinant rat IGF-I produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 71 amino acids and having a molecular mass of 7.6 kDa.
Product Specifications
Synonyms
Igf1; Igf-1; Igf-I; C730016P09Rik
NCBI Gene ID
24482
UniProt
P08025
Accession Number
NP_001075948.1
Accession Number mRNA
NM_001082479.1
Reactivity
Rat
Cross Reactivity
Rat
Sequence
MGPETLCGAELVDALQFVCGPRGFYFNKPTGYGSSIRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPTKSA
Assay Protocol
It is recommended to reconstitute the lyophilized rIGF-I in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
>98.0% as determined by SDS-PAGE
Bioactivity
The ED50 as determined by the stimulation of protein synthesis in rat L6 myoblasts was found to be < 30ng/ml corresponding to a Specific Activity of 33,334IU/mg.
Length
71
Form
Lyophilized
Reconstitution
0.4% NaHCO3
Molecular Weight
7.6 kDa
Host or Source
E. coli
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