What is the catalog number for IFN gamma?

The catalog number for IFN gamma is 100-039S.

What sizes are available for IFN gamma?

IFN gamma is available in the following size(s): 20 µg.

Is IFN gamma in stock?

IFN gamma is currently made to order / not held in immediate stock. Contact us for lead time.

IFN gamma

Interferon-gamma (IFN-gamma) is a lymphoid factor which possesses potent anti-viral activity. It has also been shown to stimulate macrophages and NK cells. Recombinant Human IFN-gamma is a 16.7 kDa protein containing 143 amino acid residues.

Product Specifications

Synonyms

IFNG; IFG; IFI

NCBI Gene ID

3458

UniProt

P01579

Accession Number

NP_000610.2

Accession Number mRNA

NM_000619

Chromosomal Location

12q14

Reactivity

Human

Cross Reactivity

Monkey, Human, Mouse, Leech, Pig

Sequence

MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ

Assay Protocol

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to ‑80°C.

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 98% by SDS-PAGE & HPLC analyses

Bioactivity

Assay #1: Determined by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 5.0-10.0 ng/ml. Assay #2: The ED50 was determined by a cytotoxicity assay using HT-29 cells is ≤ 0.05 ng/ml (> 2 x 107 units/mg) .

Length

144

Form

Lyophilized

Buffer

10mM Sodium Phosphate, pH 7.4

Reconstitution

Water

Molecular Weight

16.8 kDa

Storage Conditions

The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 12 months at -20°C to -80°C.