IFN alpha 2b

IFN-alpha is produced by macrophages and has antiviral activities. IFN stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase. IFN-a 2b Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids and having a molecular mass of 19400 Dalton. The difference between IFNA2A and IFNA2B is in the amino acid present at position 23. IFN-alpha 2a has a lysine at that position 23 while IFN-alpha 2b has arginine. The IFN-alpha 2b gene was obtained from human leukocytes.

Product Specifications

Synonyms

IFN alpha 2b, IFNA, INFA2, MGC125764, MGC125765.

NCBI Gene ID

3440

UniProt

P01563

Accession Number

NP_000596-2

Accession Number mRNA

NM_000605-3

Reactivity

Human

Cross Reactivity

Human

Assay Protocol

It is recommended to reconstitute the lyophilized IFN-alpha 2b in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Bioactivity

The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 260,000,000 IU/ mg.

Length

166

Form

Lyophilized

Reconstitution

Water

Molecular Weight

19.4 kDa

Storage Conditions

Lyophilized IFN although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN alpha 2b should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .

Host or Source

E. coli

N Terminal Sequence

MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE