ICAM-1 (CD54)

ICAMs are members of the Ig superfamily of calcium-independent transmembrane glycoproteins. ICAM-1 is a ligand for lymphocyte function-associated (LFA) and Mac-1 integrins and the major human rhinovirus receptor. The primary function of ICAM-1 is to provide adhesion between endothelial cells and leukocytes after stress or injury. The human ICAM-1 gene codes for a 505 amino acid transmembrane glycoprotein containing a 29 amino acid cytoplasmic domain, a 23 amino acid transmembrane domain, and a 453 amino acid extracellular domain. Recombinant human ICAM-1 is a 49.5 kDa glycoprotein comprising the extracellular domain (453 amino acid residues) of ICAM-1. Monomeric glycosylated ICAM-1 migrates at an apparent molecular weight of approximately 72.0-80.0 kDa by SDS-PAGE analysis under reducing conditions.

Product Specifications

Synonyms

ICAM1; BB2; CD54; P3.58

NCBI Gene ID

3383

UniProt

P05362

Accession Number

NP_000192.2

Accession Number mRNA

NM_000201

Chromosomal Location

19p13.3-p13.2

Reactivity

Anti-Human

Cross Reactivity

Human

Target Antigen

Recombinant human ICAM1 N-terminal protein

Clone

(#7D22)

Applications

ELISA, WB, IHC (Formalin/P)

Purification Method

Protein G chromatography

Assay Protocol

Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

Bioactivity

WB: 1:100-1000; IHC (Formalin/Paraffin) : 1:25-50; ELISA: Yes

Form

Lyophilized

Buffer

PBS

Reconstitution

PBS

Storage Conditions

Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.