GM-CSF

GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant murine GM-CSF is a 14.2 kDa globular protein consisting of 124 amino acids residues.

Product Specifications

Synonyms

Csf2, Csfgm; GM-CSF; Gm-CSf; MGI-IGM

NCBI Gene ID

12981

UniProt

P01587

Accession Number

NP_034099.2

Accession Number mRNA

NM_009969.4

Chromosomal Location

11 B1.3; 11 29.5 cM

Reactivity

Anti-Mouse

Cross Reactivity

Mouse

Target Antigen

Recombinant mouse GM-CSF (RT #M30-013)

Clone

Rabbit IgG

Applications

Purification Method

Protein A purified

Assay Protocol

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Bioactivity

Western Blot: use at 2-5 µg/ml.

Form

Lyophilized

Buffer

PBS

Reconstitution

Water

Storage Conditions

The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.