G-CSF

G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and mouse G-CSF are cross-species reactive. Recombinant murine G-CSF is a 19.0 kDa protein consisting of 179 amino acid residues.

Product Specifications

Synonyms

Csf3; Csfg; G-CSF; MGI-IG

NCBI Gene ID

12985

UniProt

P09920

Accession Number

NP_034101.1

Accession Number mRNA

NM_009971

Chromosomal Location

11 D; 11 57.0 cM

Reactivity

Mouse

Cross Reactivity

Mouse, Rat

Sequence

MVPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 98% by SDS-PAGE & HPLC analyses

Bioactivity

The ED50 was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.