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Flt-3 Ligand

Flt3-ligand (flk2-ligand) stimulates the proliferation and colony formation of certain BM precursor cells including CD34+ cells. Flt3-ligand produced in E. coli is a soluble non-glycosylated 17.6 kDa polypeptide containing 155 amino acid residues which comprises the extracellular domain of the transmembrane flt3-ligand protein.

Product Specifications

Synonyms

FLT3; FLK2; STK1; CD135; FLK-2

NCBI Gene ID

2323

UniProt

P49771

Accession Number

NP_001450.2

Accession Number mRNA

NM_001459.3

Chromosomal Location

19q13.3

Reactivity

Human

Cross Reactivity

Human

Sequence

TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 98% by SDS-PAGE & Coomassie stain

Bioactivity

Determined by the dose-dependent stimulation of the proliferation of human AML5 cells. The expected ED50 for this effect is 0,5 – 1 ng/mL.

Length

155

Form

Lyophilized

Molecular Weight

17.6 kDa

Host or Source

E. coli

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