FGF-10

Fibroblast Growth Factor-10 (FGF-10) is a heparin binding growth factor which stimulates the proliferation and activation of cells that express FGF receptors. FGF-10 is most related to KGF/FGF-7 and is expressed during development and preferentially in adult lungs. Human FGF-10 is a 19.3 kDa protein containing 170 amino acid residues.

Product Specifications

Synonyms

FGF10; KGF2

NCBI Gene ID

2255

UniProt

O15520

Accession Number

NP_004456.1

Accession Number mRNA

NM_004465

Chromosomal Location

5p13-p12

Reactivity

Human

Cross Reactivity

Chicken, Mouse, Human

Sequence

MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS

Assay Protocol

The lyophilized FGF-10 should be reconstituted in water to a concentration not lower than 50 μg/ml. For long term storage we would recommend to add at least 0.1% human or bovine serum albumin.

Purity

> 95% by SDS-PAGE

Bioactivity

The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF- receptors is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 106 units/mg.