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FGF-7/KGF

FGF‐7/KGF is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth, and the regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF‐7/KGF is a mitogen factor specific for epithelial cells and keratinocytes. FGF‐7/KGF signals through FGFR 2b. FGF‐7/KGF plays a role in kidney and lung development, as well as in angiogenesis and wound healing. Recombinant Murine FGF‐7/KGF is an 18.9 kDa protein consisting of 164 amino acid residues.

Product Specifications

Synonyms

Keratinocyte Growth Factor, Fibroblast Growth Factor-7, HBGF-7

NCBI Gene ID

14178

UniProt

P36363

Accession Number

NP_032034.1

Accession Number mRNA

NM_008008.4

Reactivity

Mouse

Cross Reactivity

Mouse

Sequence

MCNDMSPEQT ATSVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNSYNIMEI RTVAVGIVAI KGVESEYYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHSG GEMFVALNQK GIPVKGKKTK KEQKTAHFLP MAIT

Assay Protocol

Centrifuge the vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

≥ 95% by SDS-PAGE gel and HPLC analyses

Bioactivity

Determined by a cell proliferation assay using 4MBr-5 cells. The expected ED50 for this effect is 0.5-5.0 ng/ml.

Length

164

Form

Lyophilized

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Molecular Weight

18.9 kDa

Storage Conditions

The lyophilized protein is stable at room temperature for 3 weeks should be stored desiccated below -18°C. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.

Host or Source

E. coli

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