CCL-12 (MCP-5)

CCL12 is a cloned mouse CC chemokine most closely related to human MCP1 (66% amino acid (aa) sequence identity in the mature protein) . Mouse CCL12 encodes a 104 aa residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. The gene for mouse MCP1 has been mapped to the CC chemokine cluster on chromosome 11. Recombinant CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR2 but not CCR1, CCR3, or CCR5.

Product Specifications

Synonyms

Ccl12; MCP-5; Scya12

NCBI Gene ID

20293

UniProt

Q62401

Accession Number

NP_035461

Accession Number mRNA

NM_011331.2

Chromosomal Location

11 C; 11 47.0 cM

Reactivity

Anti-Mouse

Cross Reactivity

Mouse

Target Antigen

Recombinant mouse CCL-12

Clone

(#8J25)

Applications

WB, N

Purification Method

Protein G/A chromatography

Assay Protocol

Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

Form

Lyophilized

Buffer

PBS

Reconstitution

PBS

Storage Conditions

Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.