Amphiregulin

Amphiregulin is an EGF related growth factor that signals through the EGF/TGF-a receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts. Amphiregulin also inhibits the growth of certain carcinoma cell lines. Synthesized as a transmembrane protein, Amphiregulin’s extracellular domain is proteolytically processed to release the mature protein. There are 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds essential for biological activity. Recombinant human Amphiregulin is a 11.3 kDa glycoprotein consisting of 98 amino acid residues.

Product Specifications

Synonyms

AREG; AR; SDGF; AREGB; CRDGF

NCBI Gene ID

374

UniProt

P15514

Accession Number

NP_001648.1

Accession Number mRNA

NM_001657.2

Reactivity

Anti-Human

Cross Reactivity

Human

Target Antigen

Recombinant human Amphiregulin

Clone

Rabbit IgG

Applications

WB, IF

Purification Method

Protein A purified

Assay Protocol

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Form

Lyophilized

Buffer

PBS

Reconstitution

Water

Storage Conditions

The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.