CHAPS (hydrate)

CHAPS hydrate is a non-covalent reversible stabilizer of nucleosomes derived from Cholic Acid (HY-N0324) . CHAPS hydrate can stabilize the ultrastructure of nucleosomes, inhibit the spontaneous dissociation of nucleosomes at low concentrations, and maintain the integrity of the basic structural units of chromatin. CHAPS hydrate can promote the sliding of histone cores along the DNA chain and reduce sequence-specific binding. CHAPS hydrate can also be used as a zwitterionic detergent to dissolve membrane proteins, stabilize various protein-DNA complexes, and retain the biochemical activity of proteins in solution[1][2].

Product Specifications

CAS Number

[331717-45-4]

UNSPSC

12352200

Hazard Statement

H302, H315, H319, H335

Target

Biochemical Assay Reagents

Type

Biochemical Assay Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/chaps-hydrate.html

Purity

98.0

Solubility

DMSO : 10.87 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

O[C@H]1[C@@]2([H])[C@@]3([H])[C@@]([C@@](CC3)([H])[C@H](C)CCC(NCCC[N+](C)(C)CCCS(=O)([O-])=O)=O)([C@H](C[C@]2([H])[C@@]4([C@](C[C@@H](CC4)O)([H])C1)C)O)C.O

Molecular Formula

C32H60N2O8S

Molecular Weight

632.89

Precautions

H302, H315, H319, H335

References & Citations

[1]Kroflic A, et al. Thermodynamic characterization of 3-[ (3-cholamidopropyl) -dimethylammonium]-1-propanesulfonate (CHAPS) micellization using isothermal titration calorimetry: temperature, salt, and pH dependence. Langmuir. 2012 Jul 17;28 (28) :10363-71.|[2]Menshikova I, et al. Nucleosomes structure and dynamics: effect of CHAPS. Int J Biochem Mol Biol. 2011;2 (2) :129-137.