Anti-δ GABA-A Receptor Antibody

Mouse Monoclonal Antibody specific to δ GABA-A Receptor

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

GABA(A receptor subunit δ

Gene Name

Gabrd

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=Gabrd">Gabrd</a>

UniProt

P18506

Cellular Locus

Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Host

Mouse

Reactivity

Mouse, Rat

Immunogen

Synthetic peptide corresponding to aa 15-34 of rat Delta GABA-A receptor (accession no. P18506).

Target Antigen

γ-aminobutyric acid receptor subunit δ

Target

δ GABA-A Receptor

Clonality

Monoclonal

Isotype

IgG2a

Type

Antibody

Applications

WB, ICC/IF, AM

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

Additionnal Information

Immunoblotting: use at 1-10ug/mL. A band of ~55kDa is detected.Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/mL Immunofluorescence: use at 1-10ug/mL These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes mouse and rat Delta GABA-A receptor.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

GABA is the major inhibitory neuro- transmitter in the vertebrate brain. GABA receptors consist of five subunits that form a chloride ion channel. Subunit families alpha, beta, g, and delta have been studied extensively; Ï€ and epsilon have been identified recently. Studies of recombinant GABA receptors have shown that individual subunits and their subtypes confer different sensitivities to GABA receptor modulators. Subunit subtypes are differentially expressed throughout development and in different CNS regions, reducing the total number of possible isoforms that can be formed in different brain regions and in individual cells.