Anti-CaMKII, phosphorylated, Antibody

Mouse Monoclonal Antibody specific to CamKII, phosphorylated

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

CaM kinase II subunit α, CaMK-II subunit α, EC 2.7.11.17

Gene Name

Camk2a

NCBI Gene ID

UniProt

P11275

Cellular Locus

Cell junction, synapse, Cell junction, synapse, postsynaptic density, Cell projection, dendritic spine, Cell projection, dendrite

Host

Mouse

Reactivity

Mouse, Rat

Immunogen

Synthetic peptide based on sequence from rat/mouse CaMKII alpha (aa 281-294), thiophosphorylated at residue Thr286 conjugated to a protein carrier.

Target Antigen

Calcium/calmodulin-dependent protein kinase type II subunit α

Target

CaMKII, phosphorylated,

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF, IP, ELISA

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (PubMed:15312654). Regulates dendritic spine development. Also regulates the migration of developing neurons. Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (By similarity). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity). {UniProtKB:P11798, UniProtKB:Q9UQM7, PubMed:15312654}.

Additionnal Information

Immunoblotting: use at 1ug/mL. A band of ~50 kDa is detected. Immunofluorescence: use at 10ug/mL. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain tissue extract.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes the phosphorylated form of mouse and rat CaMKII. It does not react with non- phosphorylated CaMKII.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

CaMKII, a member of the calcium / calmodulin-activated protein kinas family, functions in neural synapatic stimulation and T-cell receptor signaling. CaMKII is expressed in many tissues, but it is specifically found in the neurons of the forebrain, and its mRNA is found within the dendrites and the soma of the neuron. CaMKII in neurons consists of two subunits of 52 (alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains as well as an ATP-binding domain and a consensus phosphorylation site. Binding of calcium / calmodulin to its regulatory domain releases its auto- inhibitory effect and activates the kinase. This kinase activation results in autophosphorylation at threonine 286. Autophosphorylation confers enhanced affinity of CaMKII for NMDA receptors in postsynaptic densities.