Anti-Human von Willebrand Factor Antibody

Mouse Monoclonal Antibody specific to von Willebrand Factor

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

vWF [Cleaved into: von Willebrand antigen 2

Gene Name

VWF

NCBI Gene ID

UniProt

P04275

Cellular Locus

Secreted, Secreted, extracellular space, extracellular matrix

Host

Mouse

Reactivity

Human

Immunogen

Human plasma von Willebrand Factor.

Target Antigen

von Willebrand factor

Target

Human von Willebrand Factor

Clonality

Monoclonal

Isotype

IgG2a

Type

Antibody

Applications

WB, IHC

Purification Method

Purified by immunoaffinity chromatography

Dilution

Reconstitute lyophilized antibody in 100ul of distilled water (final concentration 2.0mg/ml).

Format

Purified

Form

Lyophilized

Buffer

Phosphate Buffered Saline

Function

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

Additionnal Information

ELISA: 1-10ug/ml with vWF on the solid phase <br><br>Western Blot: 5-10ug/ml; useful under non-reducing conditions. <br><br>Immunohistochemistry: 5-10ug/ml on paraffin embedded and frozen tissue sections.

Storage Conditions

The stock solution (2. 0mg/ml) can be stored in aliquots at - 20°C for 1 year or can be stored at 4°C for 6 months after adding 0.1% sodium azide. Dilutions of stock solution should not be stored.

Specificity

This antibody specifically recognizes human von Willebrand Factor V8 Protease Fragment III (N-terminal trypsin and plasmin sensitive region). This antibody does not inhibit the binding of vWF to the vitronectin receptor. This antibody does not react with bovine vWF.