Recombinant Shigella flexneri Phospholipase A1 (pldA), partial
Product Specifications
Product Name Alternative
Detergent-resistant phospholipase A; DR-phospholipase A; Outer membrane phospholipase A; OM PLA; OMPLA; Phosphatidylcholine 1-acylhydrolase
Abbreviation
Recombinant Shigella flexneri pldA protein, partial
Gene Name
PldA
UniProt
P0A923
Expression Region
21-52aa
Organism
Shigella flexneri
Target Sequence
QEATVKEVHDAPAVRGSIIANMLQEHDNPFTL
Tag
N-terminal 6xHis-KSI-tagged
Type
In Stock Protein
Source
E.coli
Field of Research
Others
Relevance
Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities.
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
18.9 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Partial
Available Sizes
Frequently Asked Questions
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