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Anti-Human IgE Antibody, Alkaline Phosphatase conjugate

Goat Polyclonal Antibody specific to Human IgE-Alk Phos

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Human IgE-Alk Phos Goat Polyclonal Antibody

Host

Goat

Reactivity

Human

Immunogen

Human IgE

Target

Human IgE

Clonality

Polyclonal

Isotype

Whole IgG

Type

Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Secondary Antibodies

Purification Method

Purified by immunoaffinity chromatography

Concentration

0.5 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

HEPES

Additionnal Information

Immunoblotting: use at a dilation of 1:1,000-1:10,000. <br><br>Immunohistochemistry: use at a dilution of 1:100-1:1,000 <br><br>Immunocytochemistry: use at a dilution of 1:100-1:1,000 <br><br>ELISA: use at a dilution of 1:1,000-1:10,000. <br><br>These are recommended dilutions. <br><br>End users should determine optimal dilutions for their applications. Prepare working dilutions immediately before use.

Storage Conditions

This antibody is stable for at least 1 year at 2-8°C.

Specificity

Antibody is affinity-purified on human IgE and conjugated to alkaline phosphatase. Molar enzyme:antibody ratio = 1:1. Cross-reactivity with other immunoglobulins is negligible.

Formulation

50 mM HEPES pH 7.1, 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 containing 0.2% BSA and 0.09% NaN3

Buffer pH

pH 7.1

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