AMP-PNP (lithium hydrate)

AMP-PNP (Adenylyl imidodiphosphate) lithium hydrate is a non-hydrolyzable ATP analog. AMP-PNP lithium hydrate binds to ATP binding sites competely but is not hydrolyzed by enzymes, providing stable experimental conditions for studying ATP-dependent processes. AMP-PNP lithium hydrate can also be used to study enzyme activity, kinase regulation, DNA/RNA metabolism, ion channel function, and protein complex assembly[1][2][3][4].

Product Specifications

Product Name Alternative

Adenylyl imidodiphosphate (lithium hydrate)

UNSPSC

12352200

Target

Drug Derivative

Type

Biochemical Assay Reagents

Related Pathways

Others

Applications

Metabolism-protein/nucleotide metabolism

Field of Research

Metabolic Disease

Assay Protocol

https://www.medchemexpress.com/amp-pnp-lithium-hydrate.html

Purity

94.0

Solubility

H2O : 50 mg/mL (ultrasonic; warming)

Smiles

O[C@H]1[C@@H](O)[C@H](N2C=NC3=C2N=CN=C3N)O[C@@H]1COP(OP(NP(O)(O)=O)(O)=O)(O)=O.[x].[Li].[y].O

Molecular Formula

C10H17N6O12P3·xLi·yH2O

Molecular Weight

506.20 (free acid)

References & Citations

[1]Korkhov VM, et al. Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Nature. 2012 Oct 18;490 (7420) :367-72.|[2]Sakharov PA, et al. Nonhydrolyzable ATP analog 5'-adenylyl-imidodiphosphate (AMP-PNP) does not inhibit ATP-dependent scanning of leader sequence of mRNA. Biochemistry (Mosc) . 2015 Jan;80 (1) :45-9.|[3]Ellinghaus TL, et al. Conformational changes in the yeast mitochondrial ABC transporter Atm1 during the transport cycle. Sci Adv. 2021 Dec 24;7 (52) :eabk2392.|[4]Hehl S, Neumcke B. KATP channels of mouse skeletal muscle: mechanism of channel blockage by AMP-PNP. Eur Biophys J. 1994;23 (4) :231-7.