Anti-KCNQ4 K+ Channel Antibody

Mouse Monoclonal Antibody specific to KCNQ4 K+ Channel

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

KQT-like 4, Potassium channel subunit α KvLQT4, Voltage-gated potassium channel subunit Kv7.4

Gene Name

KCNQ4

NCBI Gene ID

UniProt

P56696

Accession Number

NP_004691.2

Cellular Locus

Basal cell membrane; Multi-pass membrane protein. Note=Situated at the basal membrane of cochlear outer hair cells.

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein aa 2-77 of human KCNQ4 (K+7.4, KvLQT4, accession number P56696).

Target Antigen

Potassium voltage-gated channel subfamily KQT member 4

Target

KCNQ4 K+ Channel

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF, IP, AM

Field of Research

Ion Channels

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Probably important in the regulation of neuronal excitability. May underlie a potassium current involved in regulating the excitability of sensory cells of the cochlea. KCNQ4 channels are blocked by linopirdin, XE991 and bepridil, whereas clofilium is without significant effect. Muscarinic agonist oxotremorine-M strongly suppress KCNQ4 current in CHO cells in which cloned KCNQ4 channels were coexpressed with M1 muscarinic receptors.

Additionnal Information

Immunoblotting: use at 1-10ug/mL. A band of ~77kDa is detected.Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/mL. Immunofluorescence: use at 1-10ug/mL These are recommended concentrations; Enduser should determine optimal concentrations for their applications. Positive control: COS cell lysate transiently expressing KCNQ4.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse and rat KCNQ4.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. The protein coded for by the KCNQ4 gene forms a potassium channel that is thought to play a critical role in the regulation of neuronal excitability, particularly in sensory cells of the cochlea. The current generated by this channel is inhibited by M1 muscarinic acetylcholine receptors and activated by retigabine, a novel anti-convulsant drug.

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