Anti-Hu CD326 Biotin

CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.

Product Specifications

Certification

RUO

Negative Species

Rat

Reactivity

Human

Immunogen

Human breast cancer MCF-7 cells

Target Antigen

CD326

Clone

323/A3

Applications

FC (QC tested), IHC-P, WB, ICC, IP

Concentration

1 mg/mL

Format

Biotin

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Kirman I, Jenkins D, Fowler R, Whelan RL: Naturally occurring antibodies to epithelial cell adhesion molecule (EpCAM) . Dig Dis Sci. 2003 Dec;48 (12) :2306-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/14714617?ordin, *Winter MJ, Nagtegaal ID, van Krieken JH, Litvínov SV: The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology. Am J Pathol. 2003 Dec;163 (6) :2139-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/14633587?ordin, *Balzar M, Briaire-de Bruijn IH, Rees-Bakker HA, Prins FA, Helfrich W, de Leij L, Riethmüller G, Alberti S, Warnaar SO, Fleuren GJ, Litvínov SV: Epidermal growth factor-like repeats mediate lateral and reciprocal interactions of Ep-CAM molecules in homophilic adhesions. Mol Cell Biol. 2001 Apr;21 (7) :2570-80., URL: https://www.ncbi.nlm.nih.gov/pubmed/11259604?ordin, *Balzar M, Bakker HA, Briaire-de-Bruijn IH, Fleuren GJ, Warnaar SO, Litvinov SV: Cytoplasmic tail regulates the intercellular adhesion function of the epithelial cell adhesion molecule. Mol Cell Biol. 1998 Aug;18 (8) :4833-43., URL: https://www.ncbi.nlm.nih.gov/pubmed/9671492?ordina, *Litvínov SV, Bakker HA, Gourevitch MM, Velders MP, Warnaar SO: Evidence for a role of the epithelial glycoprotein 40 (Ep-CAM) in epithelial cell-cell adhesion. Cell Adhes Commun. 1994 Oct;2 (5) :417-28., URL: https://www.ncbi.nlm.nih.gov/pubmed/7842256?ordina, *Litvínov SV, Velders MP, Bakker HA, Fleuren GJ, Warnaar SO: Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule. J Cell Biol. 1994 Apr;125 (2) :437-46., URL: https://www.ncbi.nlm.nih.gov/pubmed/8163559?ordina, *Edwards DP, Grzyb KT, Dressler LG, Mansel RE, Zava DT, Sledge GW Jr, McGuire WL: Monoclonal antibody identification and characterization of a Mr 43,000 membrane glycoprotein associated with human breast cancer. Cancer Res. 1986 Mar;46 (3) :1306-17., URL: https://www.ncbi.nlm.nih.gov/pubmed/3510721?ordina

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody 323/A3 recognizes an extracellular epitope of CD326 / EpCAM, a marker of epithelial lineages, that is over-expressed in many carcinomas.