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Recombinant Human FMN-dependent NADH-azoreductase (azoR)

Product Specifications

CAS Number

9000-83-3

Gene Name

azoR

UniProt

A0A2P9AIQ7

Expression Region

1-211aa

Organism

Mesorhizobium delmotII

Target Sequence

MSILLVTSSPRGNASHSTRIATELAEKLIAADPSAKLVVRDLVANPLPHIDPDYSTGIYTPVEARTQRQAEVVGVSDGVLDELFAADTIILATGFINFGISSTLKSWVDHVARSGKTFAYGENGPKGLVTGKKVYIVLASGGIYSEGAAVQMDHAIPYLRSVLGFIGMTDVDVIRIEGVGMGPEAVTAALAKASANVDAVVASTGAVAAAA

Tag

N-terminal 10xHis-tagged

Source

Yeast

Field of Research

Others

Assay Type

Developed Protein

Relevance

Also exhibits azoreductase activity. Catalyzes the reductive cleavage of the azo bond in aromatic azo compounds to the corresponding amines. Quinone reductase that provides resistance to thiol-specific stress caused by electrophilic quinones.

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Length

Full Length

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

24.3 kDa

References & Citations

Song W.-J., Kurnit D.M. Submitted (DEC-2016)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Available Sizes

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