Immobilized Papain
Product Specifications
Target
Immobilized Papain
Nature
Native
Type
Native Protein
Applications
FuncS
Field of Research
Cell Biology
Relevance
Papain is a nonspecific, cysteine-endopeptidase isolated from papaya latex that is used in a wide variety of applications. When IgG molecules are incubated with papain in the presence of cysteine, one or more peptide bonds in the hinge region are split, producing three fragments of similar size: two Fab fragments and one Fc fragment. The Fc fragments can be separated from the Fab fragments using immobilized Protein A or by ion exchange chromatography.
Purity
>95% (SDS-PAGE)
Bioactivity
Activity: 2000 units per milligram of papain before immobilization Loading: 10mg papain/g of settled gel
Form
Liquid
Storage Conditions
Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Protein Length
Full length protein
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