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Recombinant Human IL-8 (77a.a.)(rHu IL-8/CXCL8)

Product Specifications

CAS Number

9000-83-3

Synonyms

CXCL 8, Emoctakin, GCP-1, MDNCF, MONAP, NAP-1, Protein 3-10C, T-cell chemotactic factor

Source

Escherichia coli.

Purity

> 96 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human CXCR2 transfected mouse BaF3 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.

Molecular Weight

Approximately 8.9 kDa, a single, non-glycosylated polypeptide chain containing 77 amino acids.

Storage Conditions

This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

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