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Lactulose Fluorometric Assay Kit

Product Specifications

Type

Fluorometric method

Field of Research

Metabolic Diseases

Assay Principle

Lactulose (4-o-β-galactopyranosyl-D-fructo-furanose), also known as galactoside fructose, 4-β-D-galactosid-D-fructose and lactulose, contains one molecule of galactose and one molecule of fructose. Lactulose is a kind of synthetic disaccharide formed by base isomerization of lactose catalyzed by free amino group of casein during milk heat treatment. Lactulose can produce a specific product under the action of enzyme, which reacts with the chromogenic agent to produce a fluorescence product. The excitation wavelength is 530 nm, and the emission wavelength is 590 nm.

Assay Performance Time

150 min

Sample Type

Dairy product; feces

Detection Range

4.78-500 μmol/L

Sensitivity

4.78 μmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

7.4-9.7%

Intra CV

2.6-3.8%

Detection Instrument

Fluorescence Microplate Reader

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