Aphidicolin

Aphidicolin specifically inhibits DNA polymerase α and δ via binding to the enzyme, in eukaryotic cells such as the HeLa cell line, without affecting other DNA polymerases (1). Arrests cell cycle at early S phase while allowing continued cell growth(2). Potentiates apoptosis induction induced by other agents(3). Increases gene amplification frequency in HeLa S3 cells overexpressing Bcl-2(4).

(3R,4R,4aR,6aS,8R,9R,11aS,11bS) -Tetradecahydro-3,9-dihydroxy-4,11b-dimethyl-8,11a-methano-11aH-cyclohepta[a]naphthalene-4,9-dimethanol; (from Nigrospora sp.)

1. Syvaoja J., et al. (1990) Proc. Natl. Acad. Sci. USA. 87(17):6664-6668. 2. Urbani L., et al. (1995) Exp. Cell Res. 219(1):159-168. 3. Kuwakado K., et al. (1993) Biochem. Pharmacol. 46:1909-1916. 4. Yin D.X., and Schimke R.T. (1996) Proc. Natl. Acad. Sci. USA. 93:3394-3398.