PPM1H Antibody, Clone 10D2

Mouse Anti-Human PPM1H Monoclonal IgG1

Product Specifications

Background

PPM1H (Protein Phosphatase, Mg2+/Mn2+ Dependent 1H) is a serine/threonine phosphatase that modulates key signaling pathways in cellular homeostasis. It acts as a negative regulator of LRRK2 signaling by dephosphorylating Rab GTPases, which are critical for vesicle trafficking and lysosomal function. PPM1H has been identified as a gatekeeper of bone morphogenetic protein (BMP) signaling, preventing excessive activation through targeted dephosphorylation. It also serves as an independent prognostic biomarker in non-small cell lung cancer. In neuroscience, PPM1H’s regulation of Rab proteins and BMP pathways positions it as a potential modulator of neuroinflammation and synaptic integrity. Its role in maintaining vesicular transport and signaling balance makes it a candidate for therapeutic exploration in neurodegenerative diseases such as Parkinson’s.

CAS Number

9007-83-4

Specifications

Detects ~55kDa

Product Name Alternative

Protein phosphatase Mg2+/Mn2+ dependent 1H, NERPP2C, Protein phosphatase 1H, PPM1H, KIAA1157

Synonyms

PPM1H Antibody, Clone 10D2

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

57460

Swiss Prot

Q9ULR3

Accession Number

NP_065751.1

Cellular Locus

Intracellular

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Recombinant Human PPM1H Protein

Target

PPM1H

Clonality

Monoclonal

Isotype

IgG1

Clone

10D2

Conjugation

Unconjugated

Tissue Specificity

Parathyroid gland

Type

Monoclonal

Applications

WB | ICC/IF

Validated Applications

WB,ICC/IF

Field of Research

Purification

Protein G Purified

Limit Of Detection

A 1:1000 dilution of SMC-616 was sufficient for detection of PPM1H in 10 µg of Hu, Ms, Rt Kidney Cells (HEK293), Alveolar Basal Epithelial cells (A549) by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.

Concentration

1mg/ml

Dilution

WB (1:1000), ICC/IF (1:500) ; optimal dilutions for assays should be determined by the user.

Weight

0.012

Buffer

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Zhang W.Q., et al. (2021) Neoplasma. 68(5): 917-923. 2. Berndsen K., et al. (2019) 8:e50416. 3.Shen T., et al. (2014) Cell Res. 24(6): 727-741.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

-20ºC

Specificity

Detects ~55kDa

Background Reference 01

1. Zhang W.Q., et al. (2021) Neoplasma. 68 (5) : 917-923. 2. Berndsen K., et al. (2019) 8:e50416. 3.Shen T., et al. (2014) Cell Res. 24 (6) : 727-741.