DENND4C Antibody: APC

Mouse Anti-Human DENND4C Monoclonal IgG1

Product Specifications

Background

DENND4C is a guanine nucleotide exchange factor (GEF) for Rab10, a small GTPase involved in vesicle trafficking and membrane recycling. DENND4C regulates GLUT4 translocation and interacts with the retromer complex, linking it to endosomal sorting and cellular metabolism. Mutations in DENND4C have been associated with neurodevelopmental syndromes such as Alkuraya-Kucinskas and Koolen-De Vries, suggesting a broader role in brain development and function. Its involvement in membrane trafficking pathways positions DENND4C as a candidate for investigation in neurodegenerative diseases characterized by endolysosomal dysfunction.

CAS Number

9007-83-4

Specifications

Detects ~213 kDa.

Product Name Alternative

DENN domain-containing protein 4C

Synonyms

DENND4C Antibody: APC

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

55667

Swiss Prot

Q5VZ89

Accession Number

NM_017925

Cellular Locus

Cell membrane | Cytoplasm | Cytoplasmic vesicle | Membrane

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Full Length Human Recombinant Dennd4c protein

Target

DENND4C

Clonality

Monoclonal

Isotype

IgG1

Clone

3E8

Conjugation

APC

Tissue Specificity

Widely expressed

Type

Monoclonal

Applications

WB | ICC/IF | IP

Validated Applications

IHC,ICC/IF

Field of Research

Purification

Protein G Purified

Limit Of Detection

A 1:1000 dilution of SMC-610 was sufficient for detection of DENND4C in A549, MEF cells by Western Blot analysis.

Concentration

1mg/ml

Dilution

WB (1:1000) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Sano, H. et al. (2011). J Biol Chem. 286(19):16541-5. 2. https://www.genecards.org/cgi-bin/carddisp.pl?gene=DENND4C 3. McMillan, K. et al. (2016). J Cell Biol. 214(4):4389–410

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Detects ~213 kDa.

Background Reference 01

1. Sano, H. et al. (2011) . J Biol Chem. 286 (19) :16541-5. 2. https://www.genecards.org/cgi-bin/carddisp.pl?gene=DENND4C 3. McMillan, K. et al. (2016) . J Cell Biol. 214 (4) :4389–410