Tris-HCL Buffer, 10X
This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
Product Specifications
Applications
IHC
Assay Principle
1. Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or our Universal Immuno buffer.
2. The tissue slides are immersed in this 1X solution and heated for 10-20 minutes at 100ºC; OR in pressure cooker for 3-5 minutes followed by cooling at room temperature for about 20 minutes.
3. The tissue sections are ready for further IHC protocol.
pH
pH 10.0
Shipping Conditions
Blue Ice
Storage Conditions
4°C
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