Tris-HCL Buffer, 10X

This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

Product Specifications

Applications

IHC

Assay Principle

1. Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or our Universal Immuno buffer. 2. The tissue slides are immersed in this 1X solution and heated for 10-20 minutes at 100ºC; OR in pressure cooker for 3-5 minutes followed by cooling at room temperature for about 20 minutes. 3. The tissue sections are ready for further IHC protocol.