N2, N2-Dimethylguanosine

N2, N2-Dimethylguanosine is a methylated nucleoside present in RNA and a structural modification component of tRNA. N2, N2-Dimethylguanosine hinders reverse transcriptase-mediated cDNA synthesis and is one of the key modifications that affect sequencing efficiency in high-throughput RNA sequencing. N2, N2-Dimethylguanosine can selectively remove one of the methyl groups through AlkB mutant enzymes (such as D135S/L118V) and convert it into N2-methylguanosine, thereby reducing the hindrance to reverse transcription[1][2].

Product Specifications

CAS Number

[2140-67-2]

Product Name Alternative

AT2 receptor agonist C21

UNSPSC

12352005

Hazard Statement

H302, H315, H319, H335

Target

Endogenous Metabolite

Type

Natural Products

Related Pathways

Metabolic Enzyme/Protease

Applications

Cancer-programmed cell death

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/N2,N2-Dimethylguanosine.html

Purity

99.40

Solubility

DMSO : 15 mg/mL (ultrasonic)

Smiles

OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N(C=N2)C3=C2C(NC(N(C)C)=N3)=O)O1

Molecular Formula

C12H17N5O5

Molecular Weight

311.29

Precautions

H302, H315, H319, H335

References & Citations

[1]Zheng YF, et al. Urinary nucleosides as biological markers for patients with colorectal cancer. World J Gastroenterol. 2005 Jul 7;11 (25) :3871-6.|[2]Dai Q, et al. Selective Enzymatic Demethylation of N2 , N2 -Dimethylguanosine in RNA and Its Application in High-Throughput tRNA Sequencing. Angew Chem Int Ed Engl. 2017 Apr 24;56 (18) :5017-5020.