Bis-Tris
Bis-Tris is an amine buffer suitable for protein and nucleic acid systems with a pH buffer range of 5.8-7.2. Bis-Tris can also be mixed with HEPES and cacodylic acid buffer to create a pH 8 environment and monitor fluorescence emission intensity at 305 nm. Gels formulated with Bis-Tris also avoid protein degradation in samples prepared at higher pH (pH 8.5) [1][2][3].
Product Specifications
CAS Number
6976-37-0
UNSPSC
12352200
Hazard Statement
H318
Target
Biochemical Assay Reagents
Type
Biochemical Assay Reagents
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/bis-tris.html
Purity
99.91
Solubility
H2O : 200 mg/mL (ultrasonic)
Smiles
OCC(CO)(N(CCO)CCO)CO
Molecular Formula
C8H19NO5
Molecular Weight
209.24
Precautions
H318
References & Citations
[1]Oh, Soon Jin, et al. Structure and phosphodiesterase activity of Bis-Tris coordinated lanthanide (III) complexes.|[2]Hachmann JP, et al. Models of protein modification in Tris-glycine and neutral pH Bis-Tris gels during electrophoresis: effect of gel pH. Anal Biochem. 2005 Jul 15;342 (2) :237-45.|[3]Williams AS, et al. Domain 4 of the anthrax protective antigen maintains structure and binding to the host receptor CMG2 at low pH. Protein Sci. 2009 Nov;18 (11) :2277-86.
Shipping Conditions
Room Temperature
Storage Conditions
Store at room temperature, keep dry and cool
Scientific Category
Biochemical Assay Reagents
Clinical Information
No Development Reported
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
