Bis-Tris

Bis-Tris is an amine buffer suitable for protein and nucleic acid systems with a pH buffer range of 5.8-7.2. Bis-Tris can also be mixed with HEPES and cacodylic acid buffer to create a pH 8 environment and monitor fluorescence emission intensity at 305 nm. Gels formulated with Bis-Tris also avoid protein degradation in samples prepared at higher pH (pH 8.5) [1][2][3].

Product Specifications

CAS Number

[6976-37-0]

UNSPSC

12352200

Hazard Statement

H318

Target

Biochemical Assay Reagents

Type

Biochemical Assay Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/bis-tris.html

Purity

99.91

Solubility

H2O : 200 mg/mL (ultrasonic)

Smiles

OCC(CO)(N(CCO)CCO)CO

Molecular Formula

C8H19NO5

Molecular Weight

209.24

Precautions

H318

References & Citations

[1]Oh, Soon Jin, et al. Structure and phosphodiesterase activity of Bis-Tris coordinated lanthanide (III) complexes.|[2]Hachmann JP, et al. Models of protein modification in Tris-glycine and neutral pH Bis-Tris gels during electrophoresis: effect of gel pH. Anal Biochem. 2005 Jul 15;342 (2) :237-45.|[3]Williams AS, et al. Domain 4 of the anthrax protective antigen maintains structure and binding to the host receptor CMG2 at low pH. Protein Sci. 2009 Nov;18 (11) :2277-86.