Pyridoxal 5'-phosphate (hydrate)

Pyridoxal 5'-phosphate hydrate, the active form of vitamin B6, is an essential cofactor for multiple enzymes, including aromatic l-amino acid decarboxylase that catalyzes the final stage in the production of the neurotransmitters dopamine and serotonin. Pyridoxal 5'-phosphate hydrate is the most important coenzyme variant in the process of vitamin B6 intracellular phosphorylation and is interconvertible with other variants, including pyridoxine 5′‐phosphate (PNP) and pyridoxamine 5′-phosphate (PMP) [1][2].

Product Specifications

CAS Number

[853645-22-4]

Product Name Alternative

Pyridoxal phosphate (hydrate)

UNSPSC

12352005

Hazard Statement

H315, H319, H335

Target

Amyloid-β; Endogenous Metabolite; ERK

Type

Natural Products

Related Pathways

MAPK/ERK Pathway; Metabolic Enzyme/Protease; Neuronal Signaling; Stem Cell/Wnt

Applications

Metabolism-protein/nucleotide metabolism

Field of Research

Cancer; Metabolic Disease; Neurological Disease

Assay Protocol

https://www.medchemexpress.com/4-formyl-5-hydroxy-6-methylpyridin-3-yl-methyl-dihydrogen-phosphate-hydrate-1-x.html

Purity

99.76

Solubility

H2O : 2.5 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

O=CC1=C(O)C(C)=NC=C1COP(O)(O)=O.[xH2O]

Molecular Formula

C8H10NO6P

Molecular Weight

247.14

Precautions

H315, H319, H335

References & Citations

[1]Allen GF, et al. Pyridoxal 5'-phosphate deficiency causes a loss of aromatic L-amino acid decarboxylase in patients and human neuroblastoma cells, implications for aromatic L-amino acid decarboxylase and vitamin B (6) deficiency states.J Neurochem. 2010 Jul;114 (1) :87-96.|[2]Calderón-Ospina CA, et al. B Vitamins in the nervous system: Current knowledge of the biochemical modes of action and synergies of thiamine, pyridoxine, and cobalamin.CNS Neurosci Ther. 2020 Jan;26 (1) :5-13.|[3]Zhang P, et al., High concentrations of pyridoxal stimulate the expression of IGFBP1 in HepG2 cells through upregulation of the ERK/c‑Jun pathway. Mol Med Rep. 2013 Oct;8 (4) :973-8.|[4]Choi JM, et al., Membrane-Free Stem Cells and Pyridoxal 5'-Phosphate Synergistically Enhance Cognitive Function in Alzheimer's Disease Mouse Model. Antioxidants (Basel) . 2022 Mar 21;11 (3) :601. |[5]Zhang L, et al., Pyridoxine 5'-phosphate oxidase is a novel therapeutic target and regulated by the TGF-β signalling pathway in epithelial ovarian cancer. Cell Death Dis. 2017 Dec 13;8 (12) :3214.|[6]Nakamura S, et al., Pyridoxal phosphate prevents progression of diabetic nephropathy. Nephrol Dial Transplant. 2007 Aug;22 (8) :2165-74.