HRP Conjugated Digoxigenin Recombinant Rabbit Monoclonal Antibody [PSH04-39]
Selected Variant
HA722518
Size: 100 µL
Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis orientalis and Digitalis lanata (foxgloves), where it is attached to sugars, to form the glycosides (e.g. digoxin, lanatoside C). Digoxigenin is a hapten, a small molecule with high antigenicity, that is used in many molecular biology applications similarly to other popular haptens such as 2,4-Dinitrophenol, biotin, and fluorescein. Typically, digoxigenin is introduced chemically (conjugation) into biomolecules (proteins, nucleic acids) to be detected in further assays. Kd of the digoxigenin-antibody interaction has been estimated at ~12 nM (compare to Kd~0.1pM for the biotin-streptavidin interaction).
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
DIG
Host
Rabbit
Species Reactivity
Universal
Immunogen
DIG-OVA
Isotype
IgG
Clone
PSH04-39
Conjugation
HRP-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IHC-P, ELISA
Concentration
1 mg/mL
Dilution
WB: 1:2,000 ;IHC-P: 1:200-1:5,000 ;ELISA: 1:5,000-1:20,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol.
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
Price
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