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HRP Conjugated Digoxigenin Recombinant Rabbit Monoclonal Antibody [PSH04-39]

Selected Variant

HA722518

Size: 100 µL

Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis orientalis and Digitalis lanata (foxgloves), where it is attached to sugars, to form the glycosides (e.g. digoxin, lanatoside C). Digoxigenin is a hapten, a small molecule with high antigenicity, that is used in many molecular biology applications similarly to other popular haptens such as 2,4-Dinitrophenol, biotin, and fluorescein. Typically, digoxigenin is introduced chemically (conjugation) into biomolecules (proteins, nucleic acids) to be detected in further assays. Kd of the digoxigenin-antibody interaction has been estimated at ~12 nM (compare to Kd~0.1pM for the biotin-streptavidin interaction).

Product Specifications

CAS Number

9000-83-3

Product Name Alternative

1672-46-4 antibody BRN 0096479 antibody DIG antibody EINECS 216-806-2 antibody HSDB 7108 antibody Lanadigenin antibody Lanadigigenin antibody ST056392 antibody Digoxigenin

Abbreviation

DIG

Host

Rabbit

Species Reactivity

Universal

Immunogen

DIG-OVA

Isotype

IgG

Clone

PSH04-39

Conjugation

HRP-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, ELISA

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IHC-P: 1:200-1:5,000 ;ELISA: 1:5,000-1:20,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes

Price

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