Hifair™ Lyo Multiplex One Step RT-qPCR Kit

Hifair™ Lyo Multiplex One Step RT-qPCR Kit is a multiplex quantitative PCR kit based on RNA as template. In the process of the experiment, reverse transcription and quantitative PCR were carried out in the same tube, which simplified the experimental operation and reduced the risk of contamination.The unique Buffer and enzyme design ensures that it can be used in the preparation and product design of one-step RT-qPCR lyophilized reaction systems. In this kit, the first strand cDNA was efficiently synthesized by heat-resistant HifairTM V Reverse Transcriptase and quantitatively amplified by UniconTM HotStart Taq DNA Polymerase. The kit mainly contains optimized lyophilizable buffer, enzymes mix, etc. In addition, the factors that can effectively inhibit the non-specific PCR amplification and improve the amplification efficiency of multiple qPCR reactions are added, which can ensure the amplification efficiency and carry out up to multiple amplification reaction. This kit is fitted with lyoprotectant that can be used for lyophilization.