Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free, low Endotoxin (MALS verified)

T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Product Specifications

Background

Specifications

Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3G, has a calculated MW of 16.0 kDa (CD3E) and 14.4 kDa (CD3G) . Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3G contains no tag at the C-terminus. As a result of glycosylation, the protein migrates as 17-22 kDa under reducing (R) condition, and 33-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) .

Accession Number

Q95LI5-1 (CD3E) & Q95LI7-1 (CD3G)

Expression Region

Gln 22 - Asp 117 (CD3E) & Gln 23 - Thr 113 (CD3G)

Host

HEK293

Target

CD3E & CD3G

Conjugation

Unconjugated

Tag

C-10xHis | Native

Source

Cynomolgus

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.01 EU per μg

Purity

90%

Bioactivity

Immobilized Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-C52W6) at 2μg/mL (100 μL/well) can bind Bispecific BCMA×CD3 T cell-engaging Antibody with a linear range of 0.2-2 ng/mL when detected by Biotinylated Human BCMA, Fc,Avitag, premium grade (Cat. No. BC7-H82F0) (QC tested).