Recombinant Human Matrilysin (MMP7)

Product Specifications

Product Name Alternative

Matrin; Matrix metalloproteinase-7 ; MMP-7Pump-1 proteaseUterine metalloproteinase

Abbreviation

Recombinant Human MMP7 protein

Gene Name

MMP7

UniProt

P09237

Expression Region

95-267aa

Organism

Homo sapiens (Human)

Target Sequence

YSLFPNSPKWTSKVVTYRIVSYTRDLPHITVDRLVSKALNMWGKEIPLHFRKVVWGTADIMIGFARGAHGDSYPFDGPGNTLAHAFAPGTGLGGDAHFDEDERWTDGSSLGINFLYAATHELGHSLGMGHSSDPNAVMYPTYGNGDPQNFKLSQDDIKGIQKLYGKRSNSRKK

Tag

N-terminal GST-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Developmental Biology

Relevance

Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.

Molecular Weight

46.1 kDa

References & Citations

Matrilysin-inhibitor complexes common themes among metalloproteases.Browner M.F., Smith W.W., Castelhano A.L.Biochemistry 34:6602-6610 (1995)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.