Phospho-RSK1 p90 (T573) Recombinant Rabbit Monoclonal Antibody [JE50-12]
Selected Variant
HA721793
Size: 100 µL
The family of ribosomal S6 kinases (Rsks), designated Rsk-1, Rsk-2 and Rsk-3, are important signaling intermediates that mediate responses to a broad range of ligand-activated receptor tyrosine kinases. It has been established that Rsk-3 is not activated by MAP kinase in vitro, unlike Rsk-1 and Rsk-2. A unique feature common to the three members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. The Rsk family amino-terminal kinase domain is phosphorylated on Ser 227 by 3-phosphoinositide-dependent protein kinase-1 (PDK1), which increases the kinase activity of Rsk. In the carboxy-terminal kinase domain, Rsk-1 and Rsk-2 are autophosphorylated on Ser 380 and Ser 386, respectively, which mediates the docking of PDK1 to Rsk in order to promote phosphorylation of substrates, such as histone H3.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
S6K-alpha-1, p90-RSK 1, p90RSK1, p90S6K, MAPK-activated protein kinase 1a, MAPKAP kinase 1a, MAPKAPK-1a, RSK-1, RPS6KA1
Swiss Prot
Q15418 Human, P18653 Mouse, Q63531 Rat
Cellular Locus
Nucleus, Cytoplasm.
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Thr573 of human RSK1 p90.
Isotype
IgG
Clone
JE50-12
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB
Positive Control
K-562 treated with 500nM PMA for 15 minutes cell lysate, HeLa treated with 200nM PMA for 4 hours cell lysate.
Concentration
1 mg/mL
Dilution
WB: 1:1,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 83 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
Price
Frequently Asked Questions
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