OF-02

OF-02 is an ionizable lipid for mRNA delivery and a key component of lipid nanoparticles (LNPs) . OF-02 is protonated in the acidic endosomal environment to promote endosomal escape of mRNA and efficiently induce protein expression in target cells. OF-02 relies on the pH-responsive membrane fusion properties to form a complex with mRNA and destroy the endosomal membrane structure to achieve mRNA release in the cytoplasm. OF-02 is mainly used in the development of mRNA vaccines, gene editing, and protein replacement therapy[1][2][3].

Product Specifications

CAS Number

[1883431-67-1]

UNSPSC

12352005

Hazard Statement

H225, H319

Target

Liposome

Type

Oligonucleotides

Related Pathways

Metabolic Enzyme/Protease

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/of-02.html

Purity

98.0

Solubility

DMSO : 100 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

CCCCC/C=C\C/C=C\CCCCCCC(O)CN(CC(O)CCCCCC/C=C\C/C=C\CCCCC)CCCCC1C(NC(C(N1)=O)CCCCN(CC(O)CCCCCC/C=C\C/C=C\CCCCC)CC(O)CCCCCC/C=C\C/C=C\CCCCC)=O

Molecular Formula

C84H152N4O6

Molecular Weight

1314.13

Precautions

H225, H319

References & Citations

[1]Fenton OS, et al. Bioinspired Alkenyl Amino Alcohol Ionizable Lipid Materials for Highly Potent In Vivo mRNA Delivery. Adv Mater. 2016;28 (15) :2939-2943.|[2]Liu L, et al. PEGylated lipid screening, composition optimization, and structure-activity relationship determination for lipid nanoparticle-mediated mRNA delivery. Nanoscale. 2025 May 9;17 (18) :11329-11344.|[3]Fenton OS, et al. Synthesis and Biological Evaluation of Ionizable Lipid Materials for the In Vivo Delivery of Messenger RNA to B Lymphocytes. Adv Mater. 2017 Sep;29 (33) .