Anti-Hu CD1d Alexa Fluor® 647

CD1d belongs to CD1 family of transmembrane glycoproteins, associated with beta2 microglobulin, similarly to MHC I molecules. Unlike other CD1 family members, however, CD1d can be also expressed in a non-glycosylated form, which is not associated with beta2 microglobulin. Hence it is not certain how much CD1d plays a role in the presentation of microbial lipid antigens during infection. On the other hand, it is expressed on various antigen presenting cell types. Besides it, CD1d+ thymocytes are involved in the positive selection of sublineage of NKT cells.

Product Specifications

Certification

RUO

Reactivity

Human, Non-human primates

Immunogen

Human CD1d

Target Antigen

CD1d

Clone

51.1

Applications

IHC-F, WB, FC (QC tested)

Format

Alexa Fluor® 647

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Kim HS, Garcia J, Exley M, Johnson KW, Balk SP, Blumberg RS: Biochemical characterization of CD1d expression in the absence of beta2-microglobulin. J Biol Chem. 1999 Apr 2;274 (14) :9289-95., URL: https://pubmed.ncbi.nlm.nih.gov/10092605/, *Durante-Mangoni E, Wang RJ, Shaulov A, He Q, Nasser I, Afdhal N, Koziel MJ, Exley MA: Hepatic CD1d expression in hepatitis C virus infection and recognition by resident proinflammatory CD1d-reactive T cells. J Immunol. 2004 Aug 1;173 (3) :2159-66., URL: https://pubmed.ncbi.nlm.nih.gov/15265953/

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody 51.1 recognizes an extracellular epitope of CD1d, a 38 kDa transmembrane glycoprotein expressed mainly on cortical thymocytes, marginal zone B cells and other antigen presenting cells, but also i e.g. hepatitis C virus-infected livers.