Anti-Hu CD235a PE-Cy™7

CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin.

Product Specifications

Certification

RUO

Reactivity

Rat, Human

Immunogen

Membrane preparation from splenic hairy cell leukemia

Target Antigen

CD235a

Clone

JC159

Applications

FC (QC tested)

Format

PE-Cy

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Alijotas-Reig J, Palacio-Garcia C, Llurba E, Vilardell-Tarres M: Cell-derived microparticles and vascular pregnancy complications: a systematic and comprehensive review. Fertil Steril. 2013 Feb;99 (2) :441-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/23122952, *Maijenburg MW, Kleijer M, Vermeul K, Mul EP, van Alphen FP, van der Schoot CE, Voermans C: The composition of the mesenchymal stromal cell compartment in human bone marrow changes during development and aging. Haematologica. 2012 Feb;97 (2) :179-83., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=kleijer+m+vermeul+k , *Beck Z, Jagodzinski LL, Eller MA, Thelian D, Matyas GR, Kunz AN, Alving CR: Platelets and erythrocyte-bound platelets bind infectious HIV-1 in plasma of chronically infected patients. PLoS One. 2013 Nov 25;8 (11) :e81002., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=beck+z+jagodzinski+ll , *Yamauchi T, Takenaka K, Urata S, Shima T, Kikushige Y, Tokuyama T, Iwamoto C, Nishihara M, Iwasaki H, Miyamoto T, Honma N, Nakao M, Matozaki T, Akashi K: Polymorphic Sirpa is the genetic determinant for NOD-based mouse lines to achieve efficient human cell engraftment. Blood. 2013 Feb 21;121 (8) :1316-25., URL: http://www.ncbi.nlm.nih.gov/pubmed/23293079

Other References

*Nakahata T and Okumura N: Cell surface antigen expression in human erythroid progenitors: erythroid and megakaryocytic markers. Leuk Lymphoma. 1994;13: 401., URL: https://pubmed.ncbi.nlm.nih.gov/8069185/, *Yajima A, Urano-Tashiro Y, Shimazu K, Takashima E, Takahashi Y, Konishi K: Hsa, an adhesin of Streptococcus gordonii DL1, binds to alpha2-3-linked sialic acid on glycophorin A of the erythrocyte membrane. Microbiol Immunol. 2008;52 (2) :69-77., URL: https://www.ncbi.nlm.nih.gov/pubmed/18380804?ordin, *Rogers CE, Bradley MS, Palsson BO et al: Flow cytometric analysis of human bone marrow perfusion cultures: erythroid development and relationship withburst-forming units-erythroid. Exp Hematol. 1996; 24: 597., URL: https://www.ncbi.nlm.nih.gov/pubmed/8605964?dopt=A, *Bain BJ: Leukemia Diagnosis: a guide to the FAB classification. Gower Medical Publishing; 1990., URL: , *Keren DF, Hanson CA and Hurtubise PE, eds.: Flow Cytometry and Clinical Diagnosis. Chicago, IL: ASCP Press; 1994., URL:

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.