Anti-Ms CD49d PE

CD49d (integrin alpha 4), also known as very late antigen 4 alpha (VLA-4 alpha) is a cell surface glycoprotein constituting integrin complexes. CD49d heterodimerizes with CD29 (integrin beta 1) to form VLA-4 antigen, which is involved in cell-cell and cell-extracellular matrix interactions, which is a receptor for CD106 (VCAM) and fibronectin. CD79d also heterodimerizes with integrin beta 7 to form LPAM-1, which binds to MAdCAM-1 (mucosal vascular addressin) . These interactions are important for cell adhesion and activation.

Product Specifications

Certification

RUO

Reactivity

Mouse

Immunogen

Murine T lymphoma line TK1

Target Antigen

CD49d

Clone

R1-2

Applications

FC (QC tested)

Concentration

0.5 mg/mL

Format

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Zhu X, Nishimura F, Sasaki K, Fujita M, Dusak JE, Eguchi J, Fellows-Mayle W, Storkus WJ, Walker PR, Salazar AM, Okada H: Toll like receptor-3 ligand poly-ICLC promotes the efficacy of peripheral vaccinations with tumor antigen-derived peptide epitopes in murine CNS tumor models. J Transl Med. 2007 Feb 12;5:10., URL: http://www.ncbi.nlm.nih.gov/pubmed/17295916 , *Sadahira Y, Yasuda T, Yoshino T, Manabe T, Takeishi T, Kobayashi Y, Ebe Y, Naito M: Impaired splenic erythropoiesis in phlebotomized mice injected with CL2MDP-liposome: an experimental model for studying the role of stromal macrophages in erythropoiesis. J Leukoc Biol. 2000 Oct;68 (4) :464-70., URL: http://www.ncbi.nlm.nih.gov/pubmed/11037966 , *Cheung DS, Ehlenbach SJ, Kitchens RT, Riley DA, Thomas LL, Holtzman MJ, Grayson MH: Cutting edge: CD49d+ neutrophils induce FcepsilonRI expression on lung dendritic cells in a mouse model of postviral asthma. J Immunol. 2010 Nov 1;185 (9) :4983-7., URL: http://www.jimmunol.org/content/185/9/4983.long , *Geutskens SB, Nikolic T, Dardenne M, Leenen PJ, Savino W: Defective up-regulation of CD49d in final maturation of NOD mouse macrophages. Eur J Immunol. 2004 Dec;34 (12) :3465-76., URL: http://www.ncbi.nlm.nih.gov/pubmed/15517611 , *Lin J, Qin L, Chavin KD, Ding Y, Punch JD, Yang Q, Burkly LC, Bromberg JS: CD3 and CD2 ligation alters CD49d epitope expression. Pathobiology. 1995;63 (3) :119-32., URL: http://www.ncbi.nlm.nih.gov/pubmed/8821628

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The rat monoclonal antibody R1-2 recognizes an extracellular epitope of murine CD49d / Integrin alpha 4 (VLA-4 alpha), an approximately 150 kDa glycoprotein of the integrin family, expressed on multiple blood cell types.