Anti-Hu CD235a Biotin

CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin.

Product Specifications

Certification

RUO

Reactivity

Rat, Human

Immunogen

Membrane preparation from splenic hairy cell leukemia

Target Antigen

CD235a

Clone

JC159

Applications

IHC-P, IHC-F, FC (QC tested)

Concentration

1 mg/mL

Format

Biotin

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Alijotas-Reig J, Palacio-Garcia C, Llurba E, Vilardell-Tarres M: Cell-derived microparticles and vascular pregnancy complications: a systematic and comprehensive review. Fertil Steril. 2013 Feb;99 (2) :441-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/23122952, *Maijenburg MW, Kleijer M, Vermeul K, Mul EP, van Alphen FP, van der Schoot CE, Voermans C: The composition of the mesenchymal stromal cell compartment in human bone marrow changes during development and aging. Haematologica. 2012 Feb;97 (2) :179-83., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=kleijer+m+vermeul+k , *Beck Z, Jagodzinski LL, Eller MA, Thelian D, Matyas GR, Kunz AN, Alving CR: Platelets and erythrocyte-bound platelets bind infectious HIV-1 in plasma of chronically infected patients. PLoS One. 2013 Nov 25;8 (11) :e81002., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=beck+z+jagodzinski+ll , *Yamauchi T, Takenaka K, Urata S, Shima T, Kikushige Y, Tokuyama T, Iwamoto C, Nishihara M, Iwasaki H, Miyamoto T, Honma N, Nakao M, Matozaki T, Akashi K: Polymorphic Sirpa is the genetic determinant for NOD-based mouse lines to achieve efficient human cell engraftment. Blood. 2013 Feb 21;121 (8) :1316-25., URL: http://www.ncbi.nlm.nih.gov/pubmed/23293079

Other References

*Nakahata T and Okumura N: Cell surface antigen expression in human erythroid progenitors: erythroid and megakaryocytic markers. Leuk Lymphoma. 1994;13: 401., URL: https://pubmed.ncbi.nlm.nih.gov/8069185/, *Yajima A, Urano-Tashiro Y, Shimazu K, Takashima E, Takahashi Y, Konishi K: Hsa, an adhesin of Streptococcus gordonii DL1, binds to alpha2-3-linked sialic acid on glycophorin A of the erythrocyte membrane. Microbiol Immunol. 2008;52 (2) :69-77., URL: https://www.ncbi.nlm.nih.gov/pubmed/18380804?ordin, *Rogers CE, Bradley MS, Palsson BO et al: Flow cytometric analysis of human bone marrow perfusion cultures: erythroid development and relationship withburst-forming units-erythroid. Exp Hematol. 1996; 24: 597., URL: https://www.ncbi.nlm.nih.gov/pubmed/8605964?dopt=A, *Bain BJ: Leukemia Diagnosis: a guide to the FAB classification. Gower Medical Publishing; 1990., URL: , *Keren DF, Hanson CA and Hurtubise PE, eds.: Flow Cytometry and Clinical Diagnosis. Chicago, IL: ASCP Press; 1994., URL:

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.