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Anti-Hu Granzyme A Purified

Granzyme A is a serine protease expressed in the cytoplasmic granules of T cells and NK cells. Vectorial secretion of perforin and granzymes is responsible for their granule-mediated cytotoxicity. Similarly to granzyme B, granzyme A acts to destroy the target cells by proteolysis of their particular components. In case of granzyme A the targets are e.g. APEX1 (it destroys its oxidative repair activity), and nucleosome assembly protein SET (it disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA) .

Product Specifications

Certification

RUO

Immunogen

Full length human granzyme A

Target Antigen

Granzyme A

Clone

CB9

Applications

ICC, FC (QC tested), IP

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Choi BD, Gedeon PC, Herndon JE, Archer GE, Reap EA, Sanchez-Perez L, Mitchell DA, Bigner DD, Sampson JH: Human regulatory T cells kill tumor cells through granzyme-dependent cytotoxicity upon retargeting with a bispecific antibody. Cancer Immunol Res. 2013 Sep;1 (3) :163. , URL: https://pubmed.ncbi.nlm.nih.gov/24570975/, *Beresford PJ, Kam CM, Powers JC, Lieberman J: Recombinant human granzyme A binds to two putative HLA-associated proteins and cleaves one of them. Proc Natl Acad Sci U S A 1997 Aug 19;94 (17) :9285-90 , URL: https://pubmed.ncbi.nlm.nih.gov/9256474/, *Mahrus S, Craik CS: Selective chemical functional probes of granzymes A and B reveal granzyme B is a major effector of natural killer cell-mediated lysis of target cells. Chem Biol. 2005 May;12 (5) :567-77. , URL: https://pubmed.ncbi.nlm.nih.gov/15911377/, *Bratke K, Kuepper M, Bade B, Virchow JC, Luttmann W: Differential expression of human granzymes A, B, and K in natural killer cells and during CD8+ T cell differentiation in peripheral blood. Eur J Immunol. 2005 Sep;35 (9) :2608-16, URL: https://pubmed.ncbi.nlm.nih.gov/16106370/

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal CB9 recognizes granzyme A, a 28 kDa serine protease expressed intracellularly in activated Tc cells and NK cells.

Applications Notes

Flow cytometry: Recommended dilution: 5-10 μg/ml, intracellular staining.

Species

Human

CAS Number

9007-83-4

Isotype

Mouse IgG1 kappa

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