Anti-Hu C5aR2 Purified
C5aR2, also known as C5L2, is one of two receptors for C5a (anaphylatoxin) . It is coexpressed with C5aR1 (CD88) in neutrophils, as well as e.g. in mast cells, astrocytes, or macrophages, and seems to have both pro-inflammatory and anti-inflammatory roles, depending on circumstances. Unlike CD88, C5aR2 is not coupled to G-protein, thus the modulatory role is more likely.
Product Specifications
Certification
RUO
Immunogen
L1.2 cells transfected with human C5aR2
Target Antigen
C5aR2
Clone
1D9-M12
Applications
WB, FC (QC tested)
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Muenstermann M, Strobel L, Klos A, Wetsel RA, Woodruff TM, Köhl J, Johswich KO: Distinct roles of the anaphylatoxin receptors C3aR, C5aR1 and C5aR2 in experimental meningococcal infections. Virulence 2019 Dec;10 (1) :677-694., URL: https://pubmed.ncbi.nlm.nih.gov/31274379/, *Fonseca MI, McGuire SO, Counts SE, Tenner AJ: Complement activation fragment C5a receptors, CD88 and C5L2, are associated with neurofibrillary pathology. J Neuroinflammation 2013, Feb 8;10:25, URL: https://pubmed.ncbi.nlm.nih.gov/23394121/, *An LL, Gorman JV, Stephens G, Swerdlow B, Warrener P, Bonnell J, Mustelin T, Fung M, Kolbeck R: Complement C5a induces PD-L1 expression and acts in synergy with LPS through Erk1/2 and JNK signaling pathways. Sci Rep. 2016 Sep 14;6:33346., URL: https://pubmed.ncbi.nlm.nih.gov/27624143/, *Spaan AN, Vrieling M, Wallet P, Badiou C, Reyes-Robles T, Ohneck EA, Benito Y, de Haas CJ, Day CJ, Jennings MP, Lina G, Vandenesch F, van Kessel KP, Torres VJ, van Strijp JA, Henry T: The staphylococcal toxins γ-haemolysin AB and CB differentially target phagocytes by employing specific chemokine receptors. Nat Commun. 2014 Nov 11;5:5438., URL: https://pubmed.ncbi.nlm.nih.gov/25384670/, *Hao J, Wang C, Yuan J, Chen M, Zhao MH: A pro-inflammatory role of C5L2 in C5a-primed neutrophils for ANCA-induced activation . PLoS One, 2013, Jun 13;8 (6) :e66305., URL: https://pubmed.ncbi.nlm.nih.gov/23785491/
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The mouse monoclonal antibody 1D9-M12 recognizes an extracellular epitope on C5aR2 (C5L2), a C5a complement receptor, which is coexpressed with C5aR1 (CD88) in neutrophils, as well as e.g. in mast cells, astrocytes, or macrophages.
Applications Notes
Flow cytometry: Recommended dilution: 2-6 µg/ml
Species
Human, Non-human primates
CAS Number
9007-83-4
Isotype
Mouse IgG2a kappa
Curated Selection
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