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Anti-Cyclin B1 Purified

Cyclin B1 is a regulatory protein involved in mitosis. It complexes with p34 (cdc2) to form maturation-promoting factor (MPF), and is necessary for proper control of the G2/M phase transition of the cell cycle. It is expressed in tissues containing proliferating cells, such as lymph node, testis et al.

Product Specifications

Certification

RUO

Reactivity

Mammalian

Immunogen

Recombinant His-tagged hamster cyclin B1

Target Antigen

Cyclin B1

Clone

V152

Applications

FC-IC (QC tested), ICC (reported), WB (reported), IHC-P (reported)

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Yang Y, Xue K, Li Z, Zheng W, Dong W, Song J, Sun S, Ma T, Li W: c-Myc regulates the CDK1/cyclin B1 dependent‑G2/M cell cycle progression by histone H4 acetylation in Raji cells. Int J Mol Med. 2018 Jun;41 (6) :3366-3378., URL: https://www.ncbi.nlm.nih.gov/pubmed/29512702, *Wolf F, Wandke C, Isenberg N, Geley S: Dose-dependent effects of stable cyclin B1 on progression through mitosis in human cells. EMBO J. 2006 Jun 21;25 (12) :2802-13., URL: https://www.ncbi.nlm.nih.gov/pubmed/16724106, *Balsara ZR, Misaghi S, Lafave JN, Starnbach MN: Chlamydia trachomatis infection induces cleavage of the mitotic cyclin B1. Infect Immun. 2006 Oct;74 (10) :5602-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/16988235, *Geley S, Kramer E, Gieffers C, Gannon J, Peters JM, Hunt T: Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint. J Cell Biol. 2001 Apr 2;153 (1) :137-48., URL: https://pubmed.ncbi.nlm.nih.gov/11285280/, *Chan YW, Chen Y, Poon RY: Generation of an indestructible cyclin B1 by caspase-6-dependent cleavage during mitotic catastrophe. Oncogene. 2009 Jan 15;28 (2) :170-83., URL: https://www.ncbi.nlm.nih.gov/pubmed/18820706

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody V152 recognizes cyclin B1, a 48 kDa intracellular protein necessary for G2/M phase transition of the cell cycle.

Applications Notes

Flow cytometry: Recommended dilution: 2-5 µg/ml. Intracellular staining.

Isotype

Mouse IgG1

Frequently Asked Questions

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